Physical appearance Edit The Persian Sturgeon has an elongated, bulky body with a bluish tint. Populations may also occur in tributaries and rivers inbound to the Caspian Sea. Sturgeons are commercially important fishes valued for their meat but mainly for their roe. The eating habits of the sturgeon change throughout its life. As it begins life the sturgeon feeds on invertebrates including Mysid shrimp , Chironomid flies and gammarid amphipods.
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Investigation of their population structure is an important problem, the solution of which determines measures for conservation of these species. This confirms genetic isolation of the Persian sturgeon from the Russian sturgeon in the Caspian Sea.
However, in order to clarify the relationships of these species it is necessary to analyze nuclear DNA markers. The amplified fragment length polymorphism method allows estimating interpopulation and interspecific genetic distances using nuclear DNA markers.
For the amplified fragment length polymorphism AFLP method, eight pairs of selective primers were used. The analysis revealed that the Siberian sturgeon has formed a separate branch from the overall Persian-Russian sturgeons cluster, which was an expected result.
In addition, the results showed that the Caspian Russian sturgeon is closer to the Persian sturgeon from the Caspian Sea than to the Russian Sturgeon from the Sea of Azov. The present DNA marker data confirm that despite the genetic isolation of the Persian sturgeon from the Russian sturgeon in the Caspian Sea, the Persian sturgeon is a young species. These species are closely related, which has caused some difficulties with their molecular genetic identification and clarification of their phylogenetic relations.
Later, Berg called it a morphologically distinguishable subspecies of A. Research of the antigenic components of sturgeon blood serum proteins, carried out in , revealed that the Persian sturgeon is a valid sympatric species Lukyanenko et al. The taxonomic rank of A. Others find these differences indistinct and point to weakness of mitochondrial DNA marker applying for exact species identification of individuals of Russian, Persian and Adriatic A.
The Siberian sturgeon is geographically isolated from the Russian and Persian sturgeons and morphologically is easily distinguishable from them. In order to clarify the phylogenetic relations and population structure of the species within the Ponto-Caspian sturgeon clade, some authors point out the necessity to explore nuclear DNA markers Krieger et al. It should be noted that currently researchers have the opportunity to work with single nucleotide polymorphism SNP markers, which have been discovered for Ponto-Caspian sturgeons Ogden et al.
Moreover, to estimate genetic distances within the Ponto-Caspian sturgeon species group, the amplified fragment length polymorphism AFLP method is also applicable, as the AFLP technique allows to obtain a high number of dominant nuclear DNA markers Congiu et al. By examining the differences between the populations and computing genetic distances we can make suggestions on approximate time of the population separation Nei, This data analysis gives an opportunity to estimate genetic similarity of the samples, and statistically verify significance of the differences.
However, the method has some limitations. It allows to obtain a large marker set from nuclear DNA but these markers are anonymous Vos et al. This report presents the results of a molecular genetic study of interpopulation and interspecific genetic distances of the Ponto-Caspian sturgeon clade carried out with the AFLP method.
Materials and methods For this research, sturgeon tissue samples ethanol fixed fin fragments were obtained from the Russian Federal Reference Collection of Genetic Materials maintained by the Russian Federal Research Institute of Fisheries and Oceanography, Moscow, Russia.
The sample included 24 specimens of A. Next, DNA fragments were ligated with oligonucleotide adapters and used for pre-selective and selective PCR with combinations of fluorescent primers. Selective primer combinations produced sets of markers with different levels of polymorphism. The eight combinations demonstrated the most significant differentiation between samples and were selected for further analysis Table 1 : Table 1.
Physical appearance[ edit ] The Persian Sturgeon has an elongated, bulky body with a bluish tint. Populations may also occur in tributaries and rivers inbound to the Caspian Sea. Sturgeons are commercially important fishes valued for their meat but mainly for their roe. The eating habits of the sturgeon change throughout its life. As it begins life the sturgeon feeds on invertebrates including Mysid shrimp , Chironomid flies and gammarid amphipods.